DNA

Part:BBa_K4765113

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-01)
Revision as of 08:48, 9 October 2023 by Siliang Zhan (Talk | contribs) (Characterization)


Twister P1 + T7_RBS + H. ex mtSSB + stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of Hypsibius exemplaris mitochondrial single-stranded DNA binding protein (H. ex mtSSB) and ribozyme-assisted polycistronic co-expression system:pRAP. H. ex mtSSB can coat ssDNA to limit damage during desiccation and UV radiation.

Usage and Biology

We constructed SAHS 33020 into pRAP to endow E. coli with desiccation-resistant and anti-UV capability. And this composite part can cooperate with BBa_K4765112(ribozyme+T7_RBS+SAHS 33020+stem-loop) to increase the survival rate of E.coli under desiccation.

Characterization

Get details in BBa_K4765016.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 515
    Illegal BsaI.rc site found at 596
    Illegal SapI site found at 409


Reference

[edit]
Categories
//collections
Parameters
protein