Part:BBa_K4808000

Tcl40

In the biotechnological industry, temperature sensitive control systems has been widely employed, with the λpL/pR-cI system being the most common. One of its components, the pL/pR, is a temperature induced promoter originating from phage λ. Another component, cI, is a temperature sensitive repressor that can combine with the pL/pR promoter, and in result inhibiting the transcription process of the gene regulated by pL/pR promoter. Commonly, cI repressor forms a dimer and binds to pL/pR promoter, thereby inhibiting gene expression at low temperatures. Yet when temperatures rise to a certain point, the cI repressor shifts a dimer to two monomers, thus cancelling transcription inhibition. Clearly, the sensitivity to temperature of cI repressor is essential.

This year we improved BBa_C0051(wild type of cI from phage λ), Which we called TcI. We mutated TcI to create TcI40. We then tested the temperature induction threshold value of both TcI and TcI40. At a temperature of 40 degrees celcius, TcI40 loses its transcription inhibition effects, and reaches climax values of gene expression. We offer this part to future iGem teams needing a temperature sensitive control system.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]