Plasmid

Part:BBa_K4808009

Designed by: Zhao Guichun   Group: iGEM23_AIS-China   (2023-10-10)
Revision as of 11:02, 10 October 2023 by Sorata (Talk | contribs)

pTarget

pTarget plasmid that carrying specific gRNA sequence which can identity the target gene for the further gene knockout.

Characterization

pTarget plasmid play an important role in our CRISPR-CAS 9 knocking out experiment.

1280x1280.png

Step1: Transformation of the pEcCas plasmid(carry cas9 protein) into E. coli AIS-0 (CICC20905). Step2: construction of the pTarget plasmid and donor DNA. Step3: transformation of pTarget plasmid and donor DNA into the AIS-0 with pEsCas inserted. Step4: Incubation of the transformed strains.


[edit]
Categories
Parameters
None