Composite

Part:BBa_K4613024

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)
Revision as of 08:31, 10 October 2023 by Registry (Talk | contribs)

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pQE-80L-T3-MCPA

The composite part was constructed to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency. Moreover, we hope Mature Carboxypeptidase A (M-CPA), which can be found under https://parts.igem.org/Part:BBa_K4613013, can be expressed successfully in E. coli Nissle 1917 (EcN). We tried T5lac promoter from (pQE-80L).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1617
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassis
function