Composite

Part:BBa_K4613303

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)
Revision as of 07:12, 10 October 2023 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pET-29a(+)-T3-ADH3

In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried the T7lac promoter from pET-29a+. The composite part can be directly imported into plasmid and express T3-ADH3 induced with IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1007
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1742
    Illegal AgeI site found at 1430
    Illegal AgeI site found at 1592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 75


[edit]
Categories
Parameters
chassis
function