Coding

Part:BBa_K4604005

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-09-30)
Revision as of 08:49, 11 October 2023 by HannahSwientek (Talk | contribs)


BluB

Gene for BluB enzyme, catalyzes the reaction of FMNH2 to DMB used in the AdoCbl synthesis pathway in E. coli. Used in BBa_K4604015, BBa_K4604016,


Figure 1: Vitamin B12 production from precursors in E. coli. The BluB transforms the reduced form of flavin mononucleotide (FMNH2) to form 5,6-dimethylbenzimidazole (DMB) which then joins exogenously-provided cobinamide to create vitamin B12.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None