Part:BBa_K4800036

pRSFDuet-MAB4714

Carboxylic acid reductase from MMycobacterium avium.The enzyme contains an adenylation domain, a phosphopantetheinyl binding domain, and a reductase domain, and requires activation by attachment of a phosphopantetheinyl group. The enzyme activates its substrate to an adenylate form, followed by a transfer to the phosphopantetheinyl binding domain. The resulting thioester is subsequently transferred to the reductase domain, where it is reduced to an aldehyde and released.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1415
  Illegal XbaI site found at 7185
  Illegal PstI site found at 7197
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7233
  Illegal PstI site found at 7197
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 3574
  Illegal BamHI site found at 7179
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1415
  Illegal XbaI site found at 7185
  Illegal PstI site found at 7197
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1415
  Illegal XbaI site found at 7185
  Illegal PstI site found at 7197
  Illegal NgoMIV site found at 4052
  Illegal NgoMIV site found at 4354
  Illegal NgoMIV site found at 4544
  Illegal NgoMIV site found at 4907
  Illegal NgoMIV site found at 5962
  Illegal NgoMIV site found at 6341
  Illegal NgoMIV site found at 6566
  Illegal AgeI site found at 3872
  Illegal AgeI site found at 4091
  Illegal AgeI site found at 5114
  Illegal AgeI site found at 5834
  Illegal AgeI site found at 6388
  Illegal AgeI site found at 7608
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 7429
  Illegal BsaI site found at 7681
  Illegal SapI site found at 660