Coding

Part:BBa_K4865002

Designed by: Jing Shi   Group: iGEM23_OTIA-Hangzhou   (2023-08-24)
Revision as of 05:33, 1 September 2023 by Jing2023 (Talk | contribs)


PelB-EGF-R

This part generates epidermal growth factor (EGF). And it is a small protein involved in epidermal cell growth, oncogenesis, and wound healing. R is the DNA sequence of a repetitive region of Pyriform Spidroin1 (PySp1) from the piriform glandular filament of Arachnid. The function of the repeat area is mainly related to the mechanical properties of spider silk fiber. In the N-terminal is a pelB signal sequence to send the protein to periplasmic space on and then be cleaved the 5' end this also allows for potential increased stability of the folded protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 328
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 208


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Categories
Parameters
None