Coding

Part:BBa_K4879003

Designed by: Anuvind Pramod   Group: iGEM23_IISER-Pune-India   (2023-10-08)
Revision as of 18:16, 10 October 2023 by Sauron 3791 (Talk | contribs)


Yarrowia lipolytica HygR marker

Resistance marker gene for Hygromycin B, designed for expression for Yarrowia lipolytica.

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Usage and Biology

Due to the limited number of auxotrophic markers available for Yarrowia lipolytica, we had to use antibiotic-resistance markers for the selection of possible attempted deletion in our chassis. In order to use the selection marker, we had to use 200µg/ml of hygromycin B in our selection plates.

Hygromycin B tends to kill cells by interfering in polypeptide synthesis, making the organism unable to synthesise proteins. The selection marker gene, which codes for a phosphotransferase, confers resistance by modifying the hygromycin B drug molecule; making it ineffective.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None