DNA

Part:BBa_K4800011

Designed by: Yifei Wu   Group: iGEM23_NJTech-China-B   (2023-10-06)
Revision as of 16:18, 6 October 2023 by Sariel (Talk | contribs)


YcjQ Donor

YcjQ Donor is exogenous DNA used by cells as a template for homologous recombination repair (HDR) after SgRNA and Cas9 complexes have caused DNA double-strand breaks at the target site.


Document

Build:

Fig1.The genes at either end of the YcjQ gene were selected as the upper and lower homology arms

The genes at either end of the YcjQ gene were selected as the upper and lower homology arms and joined by overlap, the upper and lower homology arms were amplified separately, purified and recovered to obtain the ycjQ Donor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 631
    Illegal AgeI site found at 1313
    Illegal AgeI site found at 1321
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1373


[edit]
Categories
Parameters
None