RNA

Part:BBa_K4917011

Designed by: Mariya Roy   Group: iGEM23_Estonia-TUIT   (2023-10-05)
Revision as of 04:06, 11 October 2023 by Ssvchk (Talk | contribs) (→‎Usage and Biology)


shRNA against DWV virus ver10

Starting position for shRNA ver10 in DWV is 2104

Usage and Biology

Name  Backbone/Plasmids used for GG assembly  Content  Description  
p207pRS304pGAL1_EGFP*siRNAv10_tCYC1Plasmid containing GFP sensor fused to siRNA target
p222Golden Gate assemblyshRNA_v10shRNA expression vectors
p224Golden Gate assemblypPGK1-AGO1-tPGK1 + pTEF1-DCR1-tPGK1Plasmid containing Ago1 and <i>Dcr1 transcriptional units

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 66


[edit]
Categories
Parameters
None