Coding

Part:BBa_K4614002:Design

Designed by: Miaodi Feng   Group: iGEM23_CAU-China   (2023-09-30)
Revision as of 14:38, 5 October 2023 by FMD (Talk | contribs) (→‎References)


Lnak-R5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 529
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 529
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 529
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 529
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 405
    Illegal AgeI site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used GS linker in the design of IPN and R5 fusion proteins

Source

IPN comes from the genome of Pseudomonas syringae.

References

[1]Ping H , Poudel L , Xie H , Fang W , Zou Z , Zhai P , Wagermaier W , Fratzl P , Wang W , Wang H , O'Reilly P , Ching WY , Fu Z . Synthesis of monodisperse rod-shaped silica particles through biotemplating of surface-functionalized bacteria. Nanoscale. 2020 Apr 30;12(16):8732-8741. doi: 10.1039/d0nr00669f. PMID: 32307501.

[2]薛双红.(2017).基于细菌表面展示技术的功能性无机材料合成研究(硕士学位论文,武汉理工大学).https://kns.cnki.net/KCMS/detail/detail.aspx?dbname=CMFD201901&filename=1019808772.nh