Coding

Part:BBa_K4763007:Design

Designed by: Elizabeth Valencia Alvarez   Group: iGEM23_TecMonterreyGDL   (2023-09-25)
Revision as of 06:49, 10 October 2023 by Elyvale03 (Talk | contribs)


AtPCS (phytochelatin synthase) coding sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 649
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 649
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 649
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized sequence to disrupt the internal NdeI, NcoI and NsiI sites for convenience.


Source

Sequence was obtained from NCBI (NM_123774.4).

References