Regulatory

Part:BBa_K4604033

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-10)
Revision as of 23:36, 10 October 2023 by Registry (Talk | contribs)

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SY AdoCbl riboswitch

This Biobrick is a riboswitch for AdoCbl, a bioavailable form of B12. Upon binding to AdoCbl, a conformational change happens within the riboswitch, negatively regulating the gene downstream of it. To verify the performance of the riboswitch we created several sensors with a fluorescent readout: BBa_K4604026, BBa_K4604027, BBa_K4604028 and BBa_K4604029.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 167
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None