Composite

Part:BBa_K4724085

Designed by: Xinyao Li   Group: iGEM23_NJTech-China-A   (2023-10-10)
Revision as of 20:40, 10 October 2023 by Registry (Talk | contribs)

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TrxA-LSPETase


We fused the NusA tag to the N-terminus of LSPETase to enhance its folding characteristics and increase the solubility of the recombinant protein, thereby reducing the formation of inclusion bodies.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 322
    Illegal NgoMIV site found at 448
    Illegal AgeI site found at 535
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None