Composite

Part:BBa_K4604016

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-06)
Revision as of 16:42, 6 October 2023 by Registry (Talk | contribs)

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piG_01a (leaky_tetR_bluB)

piG_01a is a plasmid consisting of the tet promoter/repressor, the modified t7 RBS, a functional bluB gene and the rrnB terminator. The backbone we used in the experiments is pGGAselect. This version on the plasmid is leaky, a modified version of it is uploaded as BioBrick BBa_K4604015.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1647


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Parameters
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