Part:BBa_K4613002
ADH3
ADH3 is an amidohydrolase derived from Stenotrophomonas acidaminiphila and forms an octamer in solution. ADH3 was reported to exhibit 57- to 35,000-fold higher activity than other enzymes and is the most efficient OTA-detoxifying enzyme reported thus far and can hydrolyze OTA to nontoxic ochratoxin α (OTα) and L-β-phenylalanine (Phe). Moreover, soluble protein expression of ADH3 in Escherichia coli has been realized.
Fig.1 SDS-PAGE analysis of purified enzymes. M, standard protein markers; 1, purified E. coli–expressed ADH3.
Fig.2 HPLC analysis of the reaction mixtures catalyzed by WT and variant S88.
Because of its high efficiency and soluble expression in Escherichia coli , we used the variant S88E of ADH3 engineered by Xiong L et al. (2023) in our project to degrade OTA.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 733
Illegal AgeI site found at 421
Illegal AgeI site found at 583 - 1000COMPATIBLE WITH RFC[1000]
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