Composite
Part:BBa_K4724083:Design
Designed by: Xinyao Li Group: iGEM23_NJTech-China-A (2023-10-09)
DsbA-LSPETase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1186
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 322
Illegal NgoMIV site found at 442
Illegal AgeI site found at 529 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The DsbA gene sequence was inserted into the vector pET-22b (+) - LSPET-12 and a recombinant plasmid was constructed using the Gibson assembly method.
Source
An enzyme identified in the laboratory of our research group that can identify PET.