![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4604021:Design
Designed by: Hannah Swientek Group: iGEM23_Freiburg (2023-10-07)
piG_10a (tetR_bluB_riboK12_mazF)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 710
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1603
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2238
Design Notes
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Source
piG_03 (BBa_K4604019) was used as a backbone, bluB (BBa_K4604005) was inserted via Gibson Assembly.