Coding
Part:BBa_K4724073:Design
Designed by: Xinyao Li Group: iGEM23_NJTech-China-A (2023-10-07)
ompA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In the original design, because the signal peptide was a small molecule protein with a structural length of 20 to 30 amino acids, so its coding sequence was about 60 to 90 bp, we wanted to design primers to add the coding sequence of the signal peptide to the front of the coding sequence of the ISPET enzyme by PCR. We used the previously designed reverse PCR primers between the RBS and ISPET enzyme sequences to add the sequence of the signal peptide to the primers. The plasmid was subsequently constructed by homologous recombination. After insertion of the signal peptide sequence, we ensured that the signal peptide sequence homologous part was 20 bp on the forward and back primers.
Source
from E.coli