RNA
Part:BBa_K4830008:Design
Designed by: Beibitkyzy Aruzhan Group: iGEM23_NTU-Singapore (2023-10-10)
POAG snp1-3 pegRNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The design of FANCF pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail.
Source
The original FANCF pegRNA design containing spacer and rttpbs sequence was reported in Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized those and other necessary sequences, and further combined with 65777 backbone in our lab.