Part:BBa_K4815000
PYPH1 -> Pymaker generated yeast promoter High 1
Description: The part we provide is the functional promoter sequence (about 223 bp) generated by our AI model Pymaker. PYPH1 means it is predicted to be anti-mutant and to drive extremely high expression rate by our AI model.
Origin: PYPH1 targets at s. cerevisiae (saccharomyces cerevisiae), the most studied eukaryotic expression system in synthetic biology.
Loci: PYPH1 consists two parts: the core promoter and the scaffold. The core promoter is an 80 bp sequence and is seated at approximately -170 to -90 upstream to the codon (which is the presumed transcription start site-TSS and is where most transcription factors binding sites lie). The scaffold is a preserved sequence in all PYPHs. It is a structure that we learned and utilized from a previous research that can link the core promoter with the codon and provide restriction sites of BamH I and Xho I which make it possible for the plasmids with the scaffold to be inserted by various core promoter sequences at ease.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 198
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 78
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