Coding

Part:BBa_K4724011

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-06)
Revision as of 02:14, 7 October 2023 by Registry (Talk | contribs)

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IsPETaseM157A-6xHis Tag

We mutate methionine into alanine. The reason we chose this mutation is that large amino acids are mutated to small amino acids, and the active site shifts, prompting PET to better bind to the active pocket of the enzyme.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 790
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal AgeI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None