Coding

Part:BBa_K4724009

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-06)
Revision as of 00:03, 7 October 2023 by Registry (Talk | contribs)

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IsPETaseS121E-6xHis Tag

We turn serine into glutamic acid. We chose this mutation because glutamate forms hydrogen bonds with surrounding serines, prompting protein molecules to be more stable.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 790
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal AgeI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None