Coding
Part:BBa_K4724008
Designed by: Qingqing Zheng Group: iGEM23_NJTech-China-A (2023-10-06)
IsPETaseQ119F-6xHis Tag
We turn glutamine into phenylalanine. We chose this mutation because phenylalanine is a hydrophobic amino acid that promotes the formation of an active pocket hydrophobic environment. And phenylalanine has a benzene ring, which can form a large ∏ bond with PET.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 56
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 56
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 790
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 56
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 56
Illegal AgeI site found at 546 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |