Coding

Part:BBa_K4724007

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-06)
Revision as of 23:29, 6 October 2023 by Registry (Talk | contribs)

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IsPETaseT116R-6xHis Tag

We mutate threonine into arginine. We chose this mutation because PET hydrophobic surfaces have a negative charge, arginine is a basic amino acid with a positive charge, and the PET surface attracts each other.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 790
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal AgeI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None