Composite

Part:BBa_K4800041

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)
Revision as of 15:08, 8 October 2023 by Registry (Talk | contribs)

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pTrc99a-PatA

Putrescine-2-oxoglutarate transaminase from Escherichia coli. A pyridoxal 5'-phosphate protein . The product, 4-aminobutanal, spontaneously cyclizes to form 1-pyrroline, which may be the actual substrate for EC 1.2.1.19, aminobutyraldehyde dehydrogenase. Cadaverine and spermidine can also act as substrates . Forms part of the arginine-catabolism pathway

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4036
    Illegal PstI site found at 4048
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4048
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4030
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4036
    Illegal PstI site found at 4048
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4036
    Illegal PstI site found at 4048
    Illegal NgoMIV site found at 2804
    Illegal AgeI site found at 3311
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4232
    Illegal BsaI site found at 5300
    Illegal SapI.rc site found at 767


[edit]
Categories
Parameters
None