Coding
Part:BBa_K5002002
Designed by: Siqi Chen Group: iGEM23_Squirrel-Beijing-I (2023-10-10)
Cellulose degrading enzyme,cep94A+cex+cenA
Considering the fact that the discharge of printing and dyeing wastewater has a very bad impact on the environment, we wanted to reduce the content of harmful substances in printing and dyeing wastewater to the standard of discharge. Therefore, we decided construct a cellulose synthesis operon and a group of plant cellulose discomposing genes. They are derived from artificial synthesis, one can synthesize bacterial cellulose, and another can discompose the plant cellulose into glucose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 2966
Illegal NotI site found at 3943
Illegal NotI site found at 5087 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 768
Illegal BglII site found at 5000
Illegal BamHI site found at 4136
Illegal XhoI site found at 4498
Illegal XhoI site found at 4747 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 846
Illegal NgoMIV site found at 1942
Illegal NgoMIV site found at 2599
Illegal NgoMIV site found at 2972
Illegal NgoMIV site found at 3474
Illegal NgoMIV site found at 4278
Illegal NgoMIV site found at 5203 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 108
Illegal BsaI site found at 4182
Illegal BsaI.rc site found at 3019
Illegal SapI.rc site found at 3102
[edit]
Categories
Parameters
None |