Coding

Part:BBa_K5002002

Designed by: Siqi Chen   Group: iGEM23_Squirrel-Beijing-I   (2023-10-10)
Revision as of 16:14, 10 October 2023 by Registry (Talk | contribs)

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Cellulose degrading enzyme,cep94A+cex+cenA

Considering the fact that the discharge of printing and dyeing wastewater has a very bad impact on the environment, we wanted to reduce the content of harmful substances in printing and dyeing wastewater to the standard of discharge. Therefore, we decided construct a cellulose synthesis operon and a group of plant cellulose discomposing genes. They are derived from artificial synthesis, one can synthesize bacterial cellulose, and another can discompose the plant cellulose into glucose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2966
    Illegal NotI site found at 3943
    Illegal NotI site found at 5087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 768
    Illegal BglII site found at 5000
    Illegal BamHI site found at 4136
    Illegal XhoI site found at 4498
    Illegal XhoI site found at 4747
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 846
    Illegal NgoMIV site found at 1942
    Illegal NgoMIV site found at 2599
    Illegal NgoMIV site found at 2972
    Illegal NgoMIV site found at 3474
    Illegal NgoMIV site found at 4278
    Illegal NgoMIV site found at 5203
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 108
    Illegal BsaI site found at 4182
    Illegal BsaI.rc site found at 3019
    Illegal SapI.rc site found at 3102


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