RNA
Part:BBa_K4830002:Design
Designed by: Beibitkyzy Aruzhan Group: iGEM23_NTU-Singapore (2023-10-10)
RNF2 AtoC pegRNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The provided sequence contains the following parts: U6 promoter, spacer, guide RNA scaffold, rttpbs (A to C), polyA tail.
Source
The original RNF2 AtoC pegRNA sequence containing spacer and rttpbs was reported in Prime Editor 2 paper cited in References. We used those sequences and with the help of and TWIST Bioscience synthesized the parts, which were further pieced together with 65777 backbone in our lab.