DNA

Part:BBa_K4630100

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-10)
Revision as of 14:37, 10 October 2023 by James Qin (Talk | contribs) (→‎Background of the Cassette)


stgRNA-barcode-cassette 1

This part is the barcode-cassette form of the self-targeting guide RNA 1 (stgRNA 1). It is the building block of the cascade recording system. It contains Lac promoter, unique barcode, self-targeting guide RNA (stgRNA 1), double terminator and the barcode. It is used in the verification of the self-targeting and self-recombination system. The unique barcode is used to distinguish each cassette and provide unique homologous arm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 55
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 94


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Categories
Parameters
None