Part:BBa_K4417009

ureABC

Description

This part codes for the ureABC operon from Sporosarcina pasteurii, enabling the expression of the three subunits of urease enzymes.

Figure 1: ureABC operon from Sporosarcina pasteurii

Usage and Biology

• Urease (EC 3.5.1.5) is an enzyme that catalyses urea hydrolysis, forming carbon dioxide and ammonia. In biomineralization, carbonic anhydrase acts as a hydrator, catalysing the reaction of carbon dioxide (CO₂) with water into carbonic acid, which spontaneously decays into carbonate ions.
• ureABC are urease structural genes.
• Most bacterial urease has a trimer structure (ureABC)₃ of two small (ureA and ureB) and one large (ureC) subunit.

Figure 2: Urease assembly and maturation process (Veaudor T, 2019). Urease catalytic subunits (ureA, ureB, and ureC) will assemble automatically and start carbamylation, resulting in inactive apo-urease ure(ABC)3. With the presence of accessory protein, Ni will bind with apo-urease ure(ABC)₃ turning into active holo-urease.

Reference

1. Veaudor T, Cassier-Chauvat C, Chauvat F. Genomics of Urea Transport and Catabolism in Cyanobacteria: Biotechnological Implications. Front Microbiol. 2019 Sep 4;10:2052. doi: 10.3389/fmicb.2019.02052. PMID: 31551986; PMCID: PMC6737895.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]