Composite

Part:BBa_K4724022

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-10)
Revision as of 13:26, 10 October 2023 by Registry (Talk | contribs)

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IsPETase-Linker-CBM4-6xHisTag

The hydrophobicity of the PET surface prevents IsPETase from binding to PET, which prevents IsPETase from functioning well. By adding the hydrophobic domain CBM4 to IsPETase, the hydrophobic domain of IsPETase can bind to the hydrophobic surface of PET more easily under the force of water molecule movement.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 790
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal NgoMIV site found at 1079
    Illegal AgeI site found at 546
    Illegal AgeI site found at 1032
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None