Coding

Part:BBa_K4886007

Designed by: Leyu Xu   Group: iGEM23_Nanjing-BioX   (2023-10-09)
Revision as of 13:20, 10 October 2023 by Xuleyu (Talk | contribs)


F/Xpk(ASR)

This part is an ancestral F/Xpk gene predicted by ancestral sequence reconstruction (ASR), based on FXpk sequence (BBa_K4119076) derived from Clostridium acetobutylicum ATCC824. F/Xpk encodes phosphoketolase which is an enzyme with both the Fpk and Xpk activity. The enzyme catalyzes the conversion of fructose-6-phosphate (F6P) to erythrose-4-phosphate (E4P) and acetyl-phosphate (AcP), and the conversion of xylulose-5-phosphate (X5P) to glyceraldehydes-3-phosphate (G3P) and AcP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 779
    Illegal PstI site found at 1334
    Illegal PstI site found at 1928
    Illegal PstI site found at 1964
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 779
    Illegal PstI site found at 1334
    Illegal PstI site found at 1928
    Illegal PstI site found at 1964
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 779
    Illegal PstI site found at 1334
    Illegal PstI site found at 1928
    Illegal PstI site found at 1964
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 779
    Illegal PstI site found at 1334
    Illegal PstI site found at 1928
    Illegal PstI site found at 1964
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None