Composite

Part:BBa_K4653114

Designed by: Tangkun Zhu   Group: iGEM23_SZU-China   (2023-10-10)
Revision as of 12:36, 10 October 2023 by Registry (Talk | contribs)

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pMA5 - Veg promoter - SpoVG RBS - Epr - BvEP - 2x fd terminator

BvEP is an elicitor protein phosphopentomutase from Bacillus velezensis LJ02 which improves the tomato resistance to B. cinerea. High purity BvEP proteins induces the hypersensitivity response (HR) in Nicotiana tabacum. BvEP reduced the rotting rate and lesion diameter of tomato fruits caused by B. cinerea, and increased the expression of Pattern-triggered Immunity (PTI), Effector-triggered Immunity (ETI), systemic acquired resistance (SAR) related genes, ROS content, SOD and POD activities in tomato fruits, while there was no significant effect on weight loss and VC contents of tomato fruits. We designed Bacillus subtilis WB800 to express BvEP and secrete it into the environment to help tomato resist gray mold. We used pMA5 as a plasmid carrier, strong promoter Veg promoter, RBS, Epr signal peptide, BvEP expression gene, and T7TE terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 926
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1414
    Illegal BsaI.rc site found at 1482


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Categories
Parameters
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