RNA

Part:BBa_K4830000

Designed by: Beibitkyzy Aruzhan   Group: iGEM23_NTU-Singapore   (2023-10-09)
Revision as of 12:11, 10 October 2023 by Aruzhan001 (Talk | contribs)


RNF pegRNA

RNF2 pegRNA - prime editing guide RNA targeting the gene encoding Ring Finger Protein 2 (RNF), and introduces A to G (T to C) base inversion

Characterization

The pegRNA in combination with ngRNA are used to test the efficiency of the Prime Editor containing the alternative Reverse Trancriptases, by changing nucleotide at the RNF2 target site from A to G (T to C). The plasmids containing PE, pegRNA and ngRNA were trasfected into HEK293T cells, and editing efficiency was evaluated 72hr after transfection using Sanger sequencing.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/human
Parameters
biologyHuman
proteinRing Finger Protein 2