Coding

Part:BBa_K4965004

Designed by: Joseph Song   Group: iGEM23_RDFZ-CHINA   (2023-10-10)
Revision as of 11:37, 10 October 2023 by Registry (Talk | contribs)

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We added a phoA secretion tag to the hEGF gene

Although E. coli Nissle 1917 can slowly secrete the hEGF growth factor, the secretion rate is relatively low, which is not efficient enough for acne treatment. Therefore, we decided to add a phoA secretion tag to the hEGF gene, facilitating the secretion of hEGF into the periplasmic space of E. coli. phoA is a signal peptide that guides newly synthesized proteins towards the secretion pathway. hEGF is the gene responsible for synthesizing hEGF, the human epidermal growth factor. We constructed a plasmid with fused phoA-hEGF gene, transformed it into E. coli, and validated its production and secretion using ELISA and Western Blot.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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