Plasmid
Part:BBa_K4808011
Designed by: Zhao Guichun Group: iGEM23_AIS-China (2023-10-10)
pEcCas
pEcCas plasmid carry cas 9 protein which can cut the target gene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4334
Illegal EcoRI site found at 8435
Illegal EcoRI site found at 9944
Illegal EcoRI site found at 11128
Illegal EcoRI site found at 12594
Illegal XbaI site found at 7132
Illegal SpeI site found at 1124
Illegal PstI site found at 1805
Illegal PstI site found at 2760
Illegal PstI site found at 9439
Illegal PstI site found at 9686 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4334
Illegal EcoRI site found at 8435
Illegal EcoRI site found at 9944
Illegal EcoRI site found at 11128
Illegal EcoRI site found at 12594
Illegal NheI site found at 4093
Illegal NheI site found at 14303
Illegal SpeI site found at 1124
Illegal PstI site found at 1805
Illegal PstI site found at 2760
Illegal PstI site found at 9439
Illegal PstI site found at 9686
Illegal NotI site found at 1443 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4334
Illegal EcoRI site found at 8435
Illegal EcoRI site found at 9944
Illegal EcoRI site found at 11128
Illegal EcoRI site found at 12594
Illegal BglII site found at 13274
Illegal BamHI site found at 6372
Illegal BamHI site found at 8374
Illegal XhoI site found at 2613 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4334
Illegal EcoRI site found at 8435
Illegal EcoRI site found at 9944
Illegal EcoRI site found at 11128
Illegal EcoRI site found at 12594
Illegal XbaI site found at 7132
Illegal SpeI site found at 1124
Illegal PstI site found at 1805
Illegal PstI site found at 2760
Illegal PstI site found at 9439
Illegal PstI site found at 9686 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4334
Illegal EcoRI site found at 8435
Illegal EcoRI site found at 9944
Illegal EcoRI site found at 11128
Illegal EcoRI site found at 12594
Illegal XbaI site found at 7132
Illegal SpeI site found at 1124
Illegal PstI site found at 1805
Illegal PstI site found at 2760
Illegal PstI site found at 9439
Illegal PstI site found at 9686
Illegal AgeI site found at 8209
Illegal AgeI site found at 9866
Illegal AgeI site found at 13006 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 8191
[edit]
Categories
Parameters
None |