Composite

Part:BBa_K4743005:Design

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-09-16)
Revision as of 05:56, 9 October 2023 by JenL (Talk | contribs) (→‎Design Notes)


NadV+ADH1 terminator with adapter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1689
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1689
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1689
    Illegal BglII site found at 515
    Illegal BamHI site found at 16
    Illegal BamHI site found at 367
    Illegal BamHI site found at 975
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1689
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1689
    Illegal AgeI site found at 1702
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

[1] We choose the NAMPT(NadV) from Ralstonia solanacearum, because it has the highest activity compare to other organism's NAMPT.

[2] AAGGTACCGCAGGTGGGATCC is the forward primer of Adapter 1

TCACCGGTGCAGGTGGAATTC is the reverse primer of Adapter 2

Also, BamHI is included in adapter 1 and EcoRI is included in Adpater 2. These two site can be used for cloning

Source

Ralstonia solanacearum

References