Reporter

Part:BBa_K177038

Designed by: Anna Olchowik   Group: iGEM09_Warsaw   (2009-09-09)
Revision as of 00:05, 10 September 2009 by Registry (Talk | contribs)

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Bistable switch 1 testing device



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3058
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3645
    Illegal AgeI site found at 3757
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1614


[edit]
Categories
//function/reporter
//regulation/multiple
Parameters
chassisE. coli lacI deficient (i.e. Top10)
controlIPTG and 42oC
device_typebistable switch
functiontest device with GFP and RFP as reporters
negative_regulatorslacI and cIts
switch_pointPlac->cI lam switch in 42oC cI lam -> Plac in 0,1mM IPTG