Composite

Part:BBa_K4885012

Designed by: Leyu Xu   Group: iGEM23_Nanjing-SDG   (2023-10-09)
Revision as of 07:19, 10 October 2023 by Xuleyu (Talk | contribs)


Ptkt-adhE2

This part is responsible for the expression of adhE2 gene with Ptkt promotor. adhE2 gene is derived from Clostridium acetobutylicum. Ptkt is a native promoter that drives the expression of transketolase (tkt) gene in C. tyrobutyricum. This part consists of Ptkt sequence (BBa_K4886005), adhE2 sequence (BBa_K1462060), and terminator sequence (BBa_K3585002). adhE2 gene encodes alcohol/aldehyde bifunctional dehydrogenase. Its role is to convert acyl-CoA to aldehyde then to alcohol in two reductive steps using NADH as cofactor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None