Composite

Part:BBa_K4759204

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)
Revision as of 07:16, 10 October 2023 by JianghuaChen (Talk | contribs)


T7-RBS4-SUMO-linker-OleP

we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus)


Usage and Biology

Next, we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.

protein-tags.png Fig1: The application of protein tags Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 295
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 295
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 295
    Illegal BglII site found at 184
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 295
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 295
    Illegal AgeI site found at 420
    Illegal AgeI site found at 518
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None