Composite

Part:BBa_K4885008:Design

Designed by: Leyu Xu   Group: iGEM23_Nanjing-SDG   (2023-09-10)
Revision as of 06:53, 30 September 2023 by Xuleyu (Talk | contribs)


8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 214


Design Notes

In the butyrate synthesis pathway of C. tyrobutyricum L319, crotonase (crt) converts 3-hydroxybutyryl-CoA to crotonyl-CoA which is subsequently converted to butyryl-CoA by butyryl-CoA dehydrogenase coded by bcd. These two enzymes are rate-limiting enzymes. We used this part and BBa_K4885007 to express fusion protein of NGTag and crotonase (crt) and fusion protein of NGCatcher and butyryl-CoA dehydrogenase (bcd) in C. tyrobutyricum, which allows the assembly of crotonase and butyryl-CoA dehydrogenase via the NGTag/NGCatcher peptide interaction. In this way, we were able to enhance the butyrate synthesis pathway in the strain.


Source

NGTag sequence is from BBa_K4119030 crt sequence is from BBa_K4119028.

References