Composite

Part:BBa_K4885008

Designed by: Leyu Xu   Group: iGEM23_Nanjing-SDG   (2023-09-10)
Revision as of 06:53, 30 September 2023 by Xuleyu (Talk | contribs)


8

This part includes a NGTag sequence BBa_K4119030 and crt gene (BBa_K4119028) derived from Clostridium tyrobutyricum (C. tyrobutyricum) L319. It codes a fusion protein of NGTag and crotonase (crt). Crt gene codes for crotonase which converts 3-hydroxybutyryl-CoA to crotonyl-CoA in the butyrate synthesis pathway in C. tyrobutyricum. NGTag/NGCatcher peptides are variants of the SpyTag/SpyCatcher system. NGTag forms a covalent bond with NGCatcher rapidly which is not affected by the change of environment. Butyryl-CoA dehydrogenase (bcd) and crotonase (crt) modified by NGCatcher/NGTag oligopeptides can be assembled together.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 214


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Categories
Parameters
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