Composite

Part:BBa_K5023011

Designed by: Lucas Daniel Ovelar Vargas   Group: iGEM23_UNILA-LatAm   (2023-10-08)
Revision as of 01:27, 10 October 2023 by Lucasovelar (Talk | contribs)


PAR_Ble_SP7_FAST-PETase_Ter

This composite part have: PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the Fast-PETase, and the terminator. Designed to express and secrete Fast-PETase in Chlamydomonas reinhardtii, utilizing pJP32 backbone.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 704
    Illegal PstI site found at 2129
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal NheI site found at 1223
    Illegal PstI site found at 704
    Illegal PstI site found at 2129
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1634
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 704
    Illegal PstI site found at 2129
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 704
    Illegal PstI site found at 2129
    Illegal NgoMIV site found at 1085
    Illegal NgoMIV site found at 1146
    Illegal NgoMIV site found at 1438
    Illegal NgoMIV site found at 2489
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None