Composite

Part:BBa_K5023014

Designed by: Lucas Daniel Ovelar Vargas   Group: iGEM23_UNILA-LatAm   (2023-10-08)
Revision as of 21:26, 8 October 2023 by Lucasovelar (Talk | contribs)


PAR_Ble_SP7_FAST-PETase_Linker_MHETase_SP20_Ter

With the PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the PHL7 enzyme and the terminator. Designed to express and secrete PHL7 in Chlamydomonas reinhardtii, utilizing pJP32 backbone. This circuit is originally designed by our advisor João Molino.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 698
    Illegal PstI site found at 1572
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal NheI site found at 1211
    Illegal PstI site found at 698
    Illegal PstI site found at 1572
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2043
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 698
    Illegal PstI site found at 1572
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 698
    Illegal PstI site found at 1572
    Illegal NgoMIV site found at 1079
    Illegal NgoMIV site found at 1140
    Illegal NgoMIV site found at 1357
    Illegal NgoMIV site found at 1458
    Illegal NgoMIV site found at 2370
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1808


[edit]
Categories
Parameters
None