Composite

Part:BBa_K4613888:Design

Designed by: Qinfei Zhou   Group: iGEM23_NAU-CHINA   (2023-10-09)
Revision as of 20:16, 9 October 2023 by QinfeiZhou (Talk | contribs) (→‎Reference)


pSB1C3-tra-lysis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 429
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 429
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 429
    Illegal BglII site found at 14
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 429
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 429
    Illegal NgoMIV site found at 1592
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We assembled this composite part together by using In-Fusion Cloning. When designing primers, care should be taken because the sequences before Lysis E and traI are identical.


Source

References