Signalling

Part:BBa_K4719025

Designed by: Auguste Stankeviciute   Group: iGEM23_Vilnius-Lithuania   (2023-09-19)


K. xylinus extracellular signal peptide sequence

Introduction

Vilnius-Lithuania iGEM 2023 team's goal was to create synthetic biology tools for in vivo alterations of Komagataeibacter xylinus bacterial cellulose polymer composition. Firstly, we chose to produce a cellulose-chitin copolymer that would later be deacetylated, creating bacterial cellulose-chitosan. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of the bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design. As a second approach, we designed indigo-dyed cellulose that could be used as a green chemistry way to apply cellulose in the textile industry. Lastly, we have achieved a of bacterial cellulose and polyhydroxybutyrate (PHB) composite, which is synthesized by K. xylinus.


<hmtl> <body>

Usage and Biology

Proteomic analysis of K. xylinus was performed and signal peptide sequence was identified. <figure>

<img src = "output-sequence-plot.png" style = "width:400px;">
<figcaption>
Figure 1.SignalIP 6.0 output
</figcaption>

</figure>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 67
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 75
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None