Regulatory

Part:BBa_K4673029:Design

Designed by: Chieh Yu Lee   Group: iGEM23_Taipei-KCISLK-V1   (2023-10-02)
Revision as of 11:38, 2 October 2023 by Registry (Talk | contribs)

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AmpR promoter is for ampicillin resistance


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ampicillin promoter is located between T7 terminator and ampicillin resistance in the pET-22b LK vector, and located between the b-lactamase coding region and f1-origin in the pGEM-T EASY vector.


Source

GSL Biotech LLC. (2023). pET-22b(+) Sequence and Map. SnapGene. https://www.snapgene.com/plasmids/pet_and_duet_vectors_(novagen)/pET-22b(%2B)

In addition to the pET-22b-LK vector, the ampicillin promoter is also inserted into the pGEM-T EASY vector purchased from a company called Promega. https://worldwide.promega.com/products/pcr/pcr-cloning/pgem-t-easy-vector-systems/?catNum=A1360

References