Reporter
Part:BBa_K4673027:Design
Designed by: Chieh Yu Lee Group: iGEM23_Taipei-KCISLK-V1 (2023-10-02)
lacZα is a common reporter gene within E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The lacZ_α is located between the lac operon sequences and the M13-pUC_fwd_primer in the pGEM-T EASY vector.
Source
The lacZα gene was one of the components of the pGEM-T EASY vector, which our team purchased from a company called Promega. https://worldwide.promega.com/products/pcr/pcr-cloning/pgem-t-easy-vector-systems/?catNum=A1360