Coding

Part:BBa_K4891014

Designed by: Kexin Fei   Group: iGEM23_Guangzhou-MedX   (2023-06-27)
Revision as of 01:44, 20 September 2023 by KexinFei (Talk | contribs)


glk-glf

It is the key part that is responsible for strengthening glucose utilization rate. The phosphotransferase system was replaced by heterogeneously introducing glucose facilitator in strain E. coli ptsG::glk-glf.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 124
    Illegal PstI site found at 1136
    Illegal PstI site found at 1327
    Illegal PstI site found at 1900
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 124
    Illegal PstI site found at 1136
    Illegal PstI site found at 1327
    Illegal PstI site found at 1900
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 124
    Illegal PstI site found at 1136
    Illegal PstI site found at 1327
    Illegal PstI site found at 1900
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 124
    Illegal PstI site found at 1136
    Illegal PstI site found at 1327
    Illegal PstI site found at 1900
    Illegal AgeI site found at 421
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1468
    Illegal AgeI site found at 1480
    Illegal AgeI site found at 2191
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None